 The versatility and efficacy of chromatographic techniques have made them essential in both large scale and analytical separation.
Chromatography is widely used in bioprocessing to separate protein molecules from concentrated process fluids. The centre of a chromatography system is a column, filled with a media specific to the separation being carried out. How is the Kemtrak DCP007 Photometer used for chromatography product detection? Chromatography is used for protein separation and purification in biotechnology and can require one or more chromatography steps during downstream processing. Separation through chromatography requires specialized equipment to ensure maximum yield and purity. A Kemtrak DCP007-UV photometer is optimally designed for in-line real time measurement and control, allowing the detection of a protein rich solution to be diverted for collection. With an optional NIST Validation accessory mounted it will allow the user to do a validation of instrument in-line in a simple way. How does the Kemtrak DCP007 Photometer help with measuring protein levels? Advances in bioprocessing methods have led to protein products being expressed and concentrated to higher and higher levels. Higher protein concentrations mean higher absorbance and therefore, UV analysers need the ability to measure accurately at these higher absorbance levels. While absorbance measurement is essentially linear, many traditional instruments use light sources that generate a broad spectrum of wavelengths that cause stray light to enter the measurement cell resulting in a non-linear response to protein concentration, particularly at high levels. Also, traditional light sources are sensitive to temperature, generating a lot of thermal energy, and constantly drift due to deterioration. This makes them unsuitable, particularly for fragile protein molecule work. How do I install the Kemtrak DCP007 for post-column chromatography monitoring? The Kemtrak DCP007-UV analyser will deliver accurate, reliable, and repeatable post column UV and are a minimum requirement during chromatographic separation to ensure good protein fraction purity and maximum yields. With the unique Kemtrak zero dead volume measurement cell installed at the column outlet, the Kemtrak DCP007-UV analyser can provide single or dual wavelength UV absorbance analysis, in real time, for improved control of the separation process. With this, the need for offline testing and manual analyses is greatly reduced. Furthermore, the use of solid-state light sources provides the ability to measure up to 4.5 Au at 280 nm and to monitor high concentration processes up to 90 OD linearly without “peak clipping” and the undesirable effects of “hot” and powerful UV light sources on the product. Therefore, the Kemtrak DCP007-UV is the best analyser for chromatographic separation. For more information about how the Kemtrak DCP007 can assist with your chromatography, please contact us at: [email protected] or call: 01442 876777.
0 Comments
Recently, we looked at how the Kemtrak DCP007-NIR can be used to improve chromatographic separations. In this week’s post, we analyse how this popular and diverse instrument can also be used to improve solvent concentration control.
The versatility and efficacy of chromatographic techniques have made them essential in both large scale, and analytical separations. Chromatography is widely used in bio-processing to separate protein molecules from concentrated process fluids. The centre of a chromatography system is the column, filled with a media specific to the separation to be carried out. How is the technique applied? Solvent gradient control can significantly enhance peak separation and the process economy of chromatography applications. An in-line quantitative sensor permits on-line mobile phase assessment, which with controlled feedback can guarantee accurate and reproducible results. Controlled gradients minimize the effects of changing properties of the stock solvents. How was the instrument installed? Pre-Column chromatography monitoring: The Kemtrak DCP007-NIR mounted in-line before the chromatography column, continuously monitoring the solvent concentration, gives the user control over the composition delivered to the chromatography column. The online monitoring of solvent composition is more significant than measuring physical parameters like mass flow alone. As It allows the generation of batch reports that confirm the actual gradient composition and inhibits the incorrect use of a solvent. The Kemtrak hygienic measurement cells available with FDA and UPC VI approved materials, convenient zero dead volume design; contain no electronics or moving parts for ease of use. There are two versions of the Kemtrak DCP007-NIR currently available: DCP007-NIRL (850 – 1550 nm) for measurement of 0 - 100% water and solvent gradients. DCP007-NIRH (850 – 2000 nm) for trace water and hydrocarbon detection. This model incorporates a multiple stage Peltier cooled and temperature regulated photodiodes and NIR LED light source for the very best in stability and performance. For more information about this instrument and how it can help you with your chromatographic processes, please call us on: 01442 87677 or email: [email protected], where we will also be happy to provide you with a free, no obligation quote. |
Archives
August 2024
Categories
All
|
RSS Feed