Using the Kemtrak DCP007 Photometer for Chromatography Product Detection

The versatility and efficacy of chromatographic techniques have made them essential in both large scale and analytical separation.

Chromatography is widely used in bioprocessing to separate protein molecules from concentrated process fluids. The centre of a chromatography system is a column, filled with a media specific to the separation being carried out.

How is the Kemtrak DCP007 Photometer used for chromatography product detection?

Chromatography is used for protein separation and purification in biotechnology and can require one or more chromatography steps during downstream processing.

Separation through chromatography requires specialized equipment to ensure maximum yield and purity. A Kemtrak DCP007-UV photometer is optimally designed for in-line real time measurement and control, allowing the detection of a protein rich solution to be diverted for collection.

With an optional NIST Validation accessory mounted it will allow the user to do a validation of instrument in-line in a simple way.

How does the Kemtrak DCP007 Photometer help with measuring protein levels?

Advances in bioprocessing methods have led to protein products being expressed and concentrated to higher and higher levels.

Higher protein concentrations mean higher absorbance and therefore, UV analysers need the ability to measure accurately at these higher absorbance levels.

While absorbance measurement is essentially linear, many traditional instruments use light sources that generate a broad spectrum of wavelengths that cause stray light to enter the measurement cell resulting in a non-linear response to protein concentration, particularly at high levels.

Also, traditional light sources are sensitive to temperature, generating a lot of thermal energy, and constantly drift due to deterioration. This makes them unsuitable, particularly for fragile protein molecule work.

How do I install the Kemtrak DCP007 for post-column chromatography monitoring?

The Kemtrak DCP007-UV analyser will deliver accurate, reliable, and repeatable post column UV and are a minimum requirement during chromatographic separation to ensure good protein fraction purity and maximum yields.

With the unique Kemtrak zero dead volume measurement cell installed at the column outlet, the Kemtrak DCP007-UV analyser can provide single or dual wavelength UV absorbance analysis, in real time, for improved control of the separation process.

With this, the need for offline testing and manual analyses is greatly reduced. Furthermore, the use of solid-state light sources provides the ability to measure up to 4.5 Au at 280 nm and to monitor high concentration processes up to 90 OD linearly without “peak clipping” and the undesirable effects of “hot” and powerful UV light sources on the product.

Therefore, the Kemtrak DCP007-UV is the best analyser for chromatographic separation.

For more information about how the Kemtrak DCP007 can assist with your chromatography, please contact us at: [email protected] or call: 01442 876777.